Do DNA extraction methods and Taq polimerase quality improve the double repetitive element (DRE) PCR typing method for Mycobacterium tuberculosis strains?
نویسندگان
چکیده
منابع مشابه
Enterobacterial repetitive intergenic consensus sequences as molecular targets for typing of Mycobacterium tuberculosis strains.
The presence of enterobacterial repetitive intergenic consensus (ERIC) sequences was demonstrated for the first time in the genome of Mycobacterium tuberculosis; these sequences have been found in transcribed regions of the chromosomes of gram-negative bacteria. In this study genetic diversity among clinical isolates of M. tuberculosis was determined by PCR with ERIC primers (ERIC-PCR). The stu...
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The role of previous treatment in the dynamics of tuberculosis transmission has not been adequately investigated. Mycobacterium tuberculosis isolates from previously treated patients (n = 88) from all regions of Syrian Arab Republic were characterized in terms of antibiotic sensitivity and genotyping using double-repetitive-element polymerase chain reaction (DRE-PCR) method for the proximity of...
متن کاملdevelopment of a new dna extraction protocol for pfge typing of mycobacterium tuberculosis complex.
a modified pulsed-field gel electrophoresis (pfge) protocol was developed and applied to clinical isolates of mycobacterium tuberculosis complex to reduce the cost of using lyticase. this protocol reduces the expense of pfge typing of mycobacterium tuberculosis complex as it removes the use of lyticase during the spheroplast formation from these bacteria.
متن کاملA RAPID AND SIMPLE METHOD OF DNA EXTRACTION FROM CLINICAL SPECIMENS CONTAINING MYCOBACTERIUM LEPRAE FOR PCR ASSAYS
We describe a rapid, simple, and reliable procedure for routine isolation of Mycobacterium leprae DNA from slit-skin swab specimens. This one-step DNA extraction method is based on the utilization of ChelexR 100, a chelating ion exchange resin. Slit-skin swab specimens from seven untreated leprosy patients at Baba Baghi Leprosy Hospital in Iran were processed by this procedure. The polymer...
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Rao et al. (3) described a method to identify Mycobacterium tuberculosis strains of the Beijing genotype. The authors suggested using simple PCR of mycobacterial interspersed repetitive unit–variable-number tandem repeat (MIRU-VNTR) locus 26 to identify these strains. This suggestion is based on the authors’ assumption that MIRU locus 26 has a specific seven-copy signature in these strains. Rao...
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ژورنال
عنوان ژورنال: Brazilian Journal of Microbiology
سال: 2007
ISSN: 1517-8382
DOI: 10.1590/s1517-83822007000300004